Journal: Journal of Applied Crystallography

Article Title: Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures

doi: 10.1107/S1600576720013096

Figure Lengend Snippet: Images of bacteriorhodopsin crystals before cryo-FIB milling. Bright-field optical micrograph ( a ) of BR crystals grown in an LCP of monoolein at room temperature. SEM ( b ) and FIB ( c ) micrographs of a flash-frozen BR crystal with leftovers of the crystallization medium. ( d ) Geometrical features of the hexagon-shaped BR crystal are indicated by blue dashed line on the FIB image. These features can be guessed by comparing ( a ), ( b ) and ( c ). The red-dashed-line rectangle in ( d ) shows the selected area for further cryo-FIB milling. The scale bars correspond to 50 µm.

Article Snippet: Expression, purification and solubilization in β-octyl glucoside (Cube Biotech) and crystallization of bacteriorhodopsin (BR) (from Halobacterium salinarum S9 cells) in the lipidic cubic phase of monoolein (Nu-Chek Prep) were carried out as described by Gordeliy et al. (2003 ).

Techniques: Crystallization Assay